Luminescence
Equipment
The PerkinElmer LS 55 Fluorescence spectrometer offers flexibility, versatility, reliability and ease-of-use. The LS 55 includes a single cell thermostatted sample holder that can accommodate 1 cm pathlength cells as well as semi-micro cuvettes with or without stirring. Semi-micro cuvettes are available and are particularly useful where the sample is precious or volume is limited such as cell cultures and DNA samples. The system also includes an automated polarizer that consists of 2 filter wheels; each wheel containing a horizontal and vertical polarizing element. Polarizer positions are software controlled and can be manually set or automatically controlled for polarization, anisotropy or G-factor.
Measurement Modes for the LS 55: (i) Fluorescence and polarization measurement modes. (ii) Excitation, emission, constant wavelength synchronous, and constant energy synchronous spectral scanning. (iii) 3D excitation/emission scans, 3D synchronous and kinetic scans. (iv) Simple quantitation by curve fitting with a number of fit algorithms.
This instrument is also equipped with a Front Surface accessory, ideal for analyzing films, papers, powders and other flat samples. The sample may be placed in the accessory directly or held in the powder holder that has a synthetic fused silica window. Ultra small volumes or viscous samples such as oils, opaque and turbid samples may be measured via the holder or cuvette.
Fluorimeter LS 55 Perkin Elmer