Analytical and preparative chromatography
Equipment
The NIS laboratories are equipped with:
- HPLC - 10 AVP Shimadzu Diode Array: High-performance liquid chromatography (or High pressure liquid chromatography, HPLC) a powerful instrumentation used to separate, identify, and quantify different compounds.
HPLC can analyze samples over a wide polar range and is able to analyse ionic samples. Mobile phase components are selected to ensure sample solubility. Solubility in the mobile phase may preclude the analysis of some samples.
Detectors:
- Photo Diode Array (PDA) detector that can provide detection at single wavelength or multiple wavelengths. This detector also has the ability to dynamically acquire and store spectra for peak purity analysis, library searching and/or the creation of extracted signals.
- Variable Wavelength UV-Vis ideal for liquid chromatography applications from microbore to preparative scales. Electrons within unsaturated system such as double or triple bonds within organic molecule are relatively easy excited by UV radiation, and generally show absorbance within the useful UV-Visible region of the spectrum (200-600 nm). Therefore, compounds with unsaturated and aromatic characteristics generally exhibit useful absorbance spectra.
- Preparative HPLC - Shimadzu: Separation methods developed with analytical HPLC instruments can be transferred quite easily on a preparative system to perform bulk separations.
Detectors:
- Variable Wavelength UV-Vis ideal for liquid chromatography applications from microbore to preparative scales. Electrons within unsaturated system such as double or triple bonds within organic molecule are relatively easy excited by UV radiation, and generally show absorbance within the useful UV-Visible region of the spectrum (200-600 nm). Therefore, compounds with unsaturated and aromatic characteristics generally exhibit useful absorbance spectra.
- GC-MS (EI, CI, DIP) Thermo Trace MS Plus: Gas chromatography-mass spectrometry (GC-MS) is a method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
Mass spectrometric detectors have become increasingly important and are now widely used in many areas of analytical chemistry as they can be used for the detection of low analyte concentrations, for qualitative analyses or in situations where separation of analyte components is difficult.
Mass spectrometer is equipped with a single quadrupole and different ion source for Electron Impact ionization (EI) and Chemical Ionization (CI). Moreover this instrument is equipped with a probe for sample direct injection (DIP) for pure products analysis.
- Thermo Finnigan - LCQ advantage max: High-performance liquid chromatography instruments can be coupled with mass analyser detectors. Mass spectrometric detectors have become increasingly important and are now widely used in many areas of analytical chemistry as they can be used for the detection of low analyte concentrations, for qualitative analyses or in situations where separation of analyte components is difficult. The Finnigan LCQ Advantage is the workhorse ion trap mass spectrometer for high productivity LC/MS/MS results and is easily upgraded to MSn performance. The Finnigan LCQ Advantage MAX now features the universal Ion Max source that allows simple tool-less switching of ionization probes. Ion Sweep Gas reduces chemical noise, while a removable ion capillary tube allows for vent free source maintenance.
Reliability combined with an easy-to-use interface, makes the Finnigan LCQ Advantage MAX an ideal match for routine HPLC environment.
HPLC can analyze samples over a wide polar range and is able to analyse ionic samples. Mobile phase components are selected to ensure sample solubility. Solubility in the mobile phase may preclude the analysis of some samples.
- Photo Diode Array (PDA) detector that can provide detection at single wavelength or multiple wavelengths. This detector also has the ability to dynamically acquire and store spectra for peak purity analysis, library searching and/or the creation of extracted signals.
- Variable Wavelength UV-Vis ideal for liquid chromatography applications from microbore to preparative scales. Electrons within unsaturated system such as double or triple bonds within organic molecule are relatively easy excited by UV radiation, and generally show absorbance within the useful UV-Visible region of the spectrum (200-600 nm). Therefore, compounds with unsaturated and aromatic characteristics generally exhibit useful absorbance spectra.
- Variable Wavelength UV-Vis ideal for liquid chromatography applications from microbore to preparative scales. Electrons within unsaturated system such as double or triple bonds within organic molecule are relatively easy excited by UV radiation, and generally show absorbance within the useful UV-Visible region of the spectrum (200-600 nm). Therefore, compounds with unsaturated and aromatic characteristics generally exhibit useful absorbance spectra.
Mass spectrometric detectors have become increasingly important and are now widely used in many areas of analytical chemistry as they can be used for the detection of low analyte concentrations, for qualitative analyses or in situations where separation of analyte components is difficult.
Mass spectrometer is equipped with a single quadrupole and different ion source for Electron Impact ionization (EI) and Chemical Ionization (CI). Moreover this instrument is equipped with a probe for sample direct injection (DIP) for pure products analysis.
Reliability combined with an easy-to-use interface, makes the Finnigan LCQ Advantage MAX an ideal match for routine HPLC environment.